Nanite Nutrient: Follow the main path to the area with glowing green containers, then check the hidden alcove behind the large broken pipe on the left side. Interact with the container inside to collect it.
Mitochondrial Amplifier: From the Nanite Nutrient spot, climb the nearby ladder to the upper level, cross the narrow catwalk, enter the room with the flickering light, and grab the item from the shelf in the back corner.
Both items are obtainable during normal progression — no backtracking or sequence breaks required.
Why These Two Items Matter More Than You Think
The Last Caretaker doesn’t hold your hand when it comes to progression items. You can walk past both the Nanite Nutrient and the Mitochondrial Amplifier without realizing they’re mandatory, and then hit a wall twenty minutes later wondering what you missed. I’ve seen the confusion firsthand in community threads — players circling the same rooms, assuming a puzzle is broken when the real issue is a missed pickup.

The good news? Both items sit along the critical path. You don’t need to detour into optional zones or solve obscure environmental puzzles. You just need to know exactly what to look for, because neither item announces itself with a marker or a UI prompt.
Where I Find the Nanite Nutrient
The Nanite Nutrient is the first of the two items you’ll encounter, and it’s the easier one to miss. Here’s the exact route I take:
- Follow the main path until you reach the area with the glowing green containers. This is a distinct visual landmark — you can’t mistake it once you’re there.
- Look for a hidden alcove behind the large broken pipe on the left side. The pipe reads as set dressing, so most players walk straight past it.
- Interact with the container inside the alcove to collect the Nanite Nutrient.
The key detail here is the broken pipe. It blends into the industrial environment, and there’s no visual indicator that anything sits behind it. If you’re the type of player who sticks to the center of corridors, you will miss this. My rule: whenever you see a large obstruction on the side of a main path in The Last Caretaker, check behind it. The game hides progression items in these negative spaces deliberately.
Tip: If you’ve already passed the green container area, don’t panic. The alcove doesn’t lock you out — you can double back at any point before the next major story beat.
How I Grab the Mitochondrial Amplifier
Once the Nanite Nutrient is in your inventory, the Mitochondrial Amplifier is only a short climb away. This one is less hidden but requires you to commit to a vertical detour:
- From the Nanite Nutrient location, proceed to the upper level via the nearby ladder. It’s directly adjacent to the alcove area.
- Cross the narrow catwalk and enter the room with the flickering light. The flicker is your cue — it marks the correct doorway.
- The Mitochondrial Amplifier is on a shelf in the back corner — pick it up.
The narrow catwalk is the section where most players hesitate. It looks like a traversal puzzle, and the flickering light reads as a hazard warning. It’s neither. The catwalk is safe, and the light is just a breadcrumb pointing you toward the correct room.
If early progression is the actual blocker, I switch to the How to Get Calcium — calcium is another key material you’ll need soon after securing these two items.
My Route Summary
| Step | Item | Location | Key Visual Cue |
|---|---|---|---|
| 1 | Nanite Nutrient | Hidden alcove behind broken pipe | Glowing green containers |
| 2 | Mitochondrial Amplifier | Shelf in back corner | Flickering light, narrow catwalk |
Both pickups take under five minutes total once you know where to look. The real time sink is reconnaissance — which is exactly why I wrote this down.
Once you have both items, you’ll likely need additional materials for crafting. If I need to know what to do next, I use the How to Get Omega 3 to plan my next gathering session.
More Guides:
- The Last Caretaker: How to Get Calcium
- The Last Caretaker: How to Get Vitamin D
- The Last Caretaker: How to Get Omega 3
- The Last Caretaker: How to Farm Protein and Carbohydrate Easily
- The Last Caretaker: How to Craft Fat